proco2 carbon dioxide sensor Search Results


pro co2 carbon dioxide controller  (BioSpherix)
96
BioSpherix pro co2 carbon dioxide controller
Fig. 2. High <t>CO2</t> increases ACh-induced cell contraction via miR-133a–RhoA axis signaling in human ASM cells. All experiments were performed in human ASM (hASM) cells exposed to 5% (Ctrl) or 20% CO2 for 2 days. (A) Representative images (top) and quantification (bottom) of ACh-induced cell contraction (n = 8 cells). Scale bars, 50 m (top). (B) Representative images (top) and quantification (bottom) of ACh-induced cell contraction in human ASM cells treated with or without 1 M Y-27632 (n = 8 cells). Scale bars, 50 m (top). (C) Representative images (top) and quantification (bottom) of ACh-induced cell contraction in human ASM cells with lentiviral over- expression of control miR (Ctrl miR) or miR-133 (n = 8 cells). Scale bars, 50 m (top). All data are expressed as median with interquartile range. Statistical analysis was performed by Kruskal-Wallis with Dunn’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001.
Pro Co2 Carbon Dioxide Controller, supplied by BioSpherix, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
pro co2 carbon dioxide controller - by Bioz Stars, 2026-05
96/100 stars
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oxygen controller device  (BioSpherix)
96
BioSpherix oxygen controller device
Fig. 2. High <t>CO2</t> increases ACh-induced cell contraction via miR-133a–RhoA axis signaling in human ASM cells. All experiments were performed in human ASM (hASM) cells exposed to 5% (Ctrl) or 20% CO2 for 2 days. (A) Representative images (top) and quantification (bottom) of ACh-induced cell contraction (n = 8 cells). Scale bars, 50 m (top). (B) Representative images (top) and quantification (bottom) of ACh-induced cell contraction in human ASM cells treated with or without 1 M Y-27632 (n = 8 cells). Scale bars, 50 m (top). (C) Representative images (top) and quantification (bottom) of ACh-induced cell contraction in human ASM cells with lentiviral over- expression of control miR (Ctrl miR) or miR-133 (n = 8 cells). Scale bars, 50 m (top). All data are expressed as median with interquartile range. Statistical analysis was performed by Kruskal-Wallis with Dunn’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001.
Oxygen Controller Device, supplied by BioSpherix, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/oxygen controller device/product/BioSpherix
Average 96 stars, based on 1 article reviews
oxygen controller device - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier

Image Search Results


Fig. 2. High CO2 increases ACh-induced cell contraction via miR-133a–RhoA axis signaling in human ASM cells. All experiments were performed in human ASM (hASM) cells exposed to 5% (Ctrl) or 20% CO2 for 2 days. (A) Representative images (top) and quantification (bottom) of ACh-induced cell contraction (n = 8 cells). Scale bars, 50 m (top). (B) Representative images (top) and quantification (bottom) of ACh-induced cell contraction in human ASM cells treated with or without 1 M Y-27632 (n = 8 cells). Scale bars, 50 m (top). (C) Representative images (top) and quantification (bottom) of ACh-induced cell contraction in human ASM cells with lentiviral over- expression of control miR (Ctrl miR) or miR-133 (n = 8 cells). Scale bars, 50 m (top). All data are expressed as median with interquartile range. Statistical analysis was performed by Kruskal-Wallis with Dunn’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001.

Journal: Science translational medicine

Article Title: Hypercapnia increases airway smooth muscle contractility via caspase-7-mediated miR-133a-RhoA signaling.

doi: 10.1126/scitranslmed.aat1662

Figure Lengend Snippet: Fig. 2. High CO2 increases ACh-induced cell contraction via miR-133a–RhoA axis signaling in human ASM cells. All experiments were performed in human ASM (hASM) cells exposed to 5% (Ctrl) or 20% CO2 for 2 days. (A) Representative images (top) and quantification (bottom) of ACh-induced cell contraction (n = 8 cells). Scale bars, 50 m (top). (B) Representative images (top) and quantification (bottom) of ACh-induced cell contraction in human ASM cells treated with or without 1 M Y-27632 (n = 8 cells). Scale bars, 50 m (top). (C) Representative images (top) and quantification (bottom) of ACh-induced cell contraction in human ASM cells with lentiviral over- expression of control miR (Ctrl miR) or miR-133 (n = 8 cells). Scale bars, 50 m (top). All data are expressed as median with interquartile range. Statistical analysis was performed by Kruskal-Wallis with Dunn’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 carbon dioxide controller (BioSpherix).

Techniques: Over Expression, Control

Fig. 4. High CO2 induces CASPASE-7–dependent cleavage of MEF2D in human ASM cells. Representative West- ern blot (top) and quantification (bottom) of cleaved CASPASE-7 (n = 4) and MEF2D (n = 4) in nuclear fraction (A) and procaspase-7 (n = 3) in whole-cell lysate (B) in human ASM cells. All data are expressed as median with interquartile range. Statistical analysis was performed by Mann-Whitney U test. *P < 0.05.

Journal: Science translational medicine

Article Title: Hypercapnia increases airway smooth muscle contractility via caspase-7-mediated miR-133a-RhoA signaling.

doi: 10.1126/scitranslmed.aat1662

Figure Lengend Snippet: Fig. 4. High CO2 induces CASPASE-7–dependent cleavage of MEF2D in human ASM cells. Representative West- ern blot (top) and quantification (bottom) of cleaved CASPASE-7 (n = 4) and MEF2D (n = 4) in nuclear fraction (A) and procaspase-7 (n = 3) in whole-cell lysate (B) in human ASM cells. All data are expressed as median with interquartile range. Statistical analysis was performed by Mann-Whitney U test. *P < 0.05.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 carbon dioxide controller (BioSpherix).

Techniques: MANN-WHITNEY

Fig. 5. High CO2 activates calcium-calpain signaling, leading to Caspase-7 activation. (A) Time course of calpain activity in mouse ASM cells exposed to 5% (Ctrl) or 20% CO2 (Ctrl, n = 30; 20% CO2, n = 6 per group). (B) Representative Western blot (top) and quantification (bottom) of Calpain-1 and Calpain-2 in ASM cells exposed to Ctrl or 20% CO2 for 6 hours (Ctrl, n = 8; 20% CO2, n = 4 per group). (C) Representative Western blot (top) and quantification (bottom) of cleaved Caspase-7 in ASM cells trans- fected with si-Scr, Calpain-1 (si-Capn1), or Calpain-2 (si-Capn2) and exposed to Ctrl or 20% CO2 for 2 days (Ctrl si-Scr, n = 8; others, n = 4 per group). (D) Intracellular Ca2+ in ASM cells exposed to Ctrl or 20% CO2 for 6 hours (n = 7 cells). (E) Representative Ca2+ oscillation induced by ACh in ASM cells exposed to Ctrl or 20% CO2 for 6 hours (n = 7 cells). (F) Calpain activity in ASM cells treated with or without 1 M BAPTA-AM and exposed to Ctrl or 20% CO2 for 6 hours (n = 6). All data are expressed as means ± SEM. Statistical analysis was performed by one-way ANOVA with Dunnett’s post hoc test (A and B) or with Tukey’s post hoc test (C and F). For (A) and (B), all comparisons were made with the control group. *P < 0.05, **P < 0.01, ***P < 0.001.

Journal: Science translational medicine

Article Title: Hypercapnia increases airway smooth muscle contractility via caspase-7-mediated miR-133a-RhoA signaling.

doi: 10.1126/scitranslmed.aat1662

Figure Lengend Snippet: Fig. 5. High CO2 activates calcium-calpain signaling, leading to Caspase-7 activation. (A) Time course of calpain activity in mouse ASM cells exposed to 5% (Ctrl) or 20% CO2 (Ctrl, n = 30; 20% CO2, n = 6 per group). (B) Representative Western blot (top) and quantification (bottom) of Calpain-1 and Calpain-2 in ASM cells exposed to Ctrl or 20% CO2 for 6 hours (Ctrl, n = 8; 20% CO2, n = 4 per group). (C) Representative Western blot (top) and quantification (bottom) of cleaved Caspase-7 in ASM cells trans- fected with si-Scr, Calpain-1 (si-Capn1), or Calpain-2 (si-Capn2) and exposed to Ctrl or 20% CO2 for 2 days (Ctrl si-Scr, n = 8; others, n = 4 per group). (D) Intracellular Ca2+ in ASM cells exposed to Ctrl or 20% CO2 for 6 hours (n = 7 cells). (E) Representative Ca2+ oscillation induced by ACh in ASM cells exposed to Ctrl or 20% CO2 for 6 hours (n = 7 cells). (F) Calpain activity in ASM cells treated with or without 1 M BAPTA-AM and exposed to Ctrl or 20% CO2 for 6 hours (n = 6). All data are expressed as means ± SEM. Statistical analysis was performed by one-way ANOVA with Dunnett’s post hoc test (A and B) or with Tukey’s post hoc test (C and F). For (A) and (B), all comparisons were made with the control group. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 carbon dioxide controller (BioSpherix).

Techniques: Activation Assay, Activity Assay, Western Blot, Control